DETECTION OF NITROGENASE PRODUCING BACTERIA FROM THE SOIL OF LIWA BOTANICAL GARDEN

Liwa Botanical Gardens is an ex-situ conservation area for various types of plants. Each plant produces organic matter that will provide nutrients the growth nitrogen-fixing bacteria. This indicates existence environment supports Nitrogen one needed by plants their growth. However, abundance nitrogen in atmosphere cannot be utilized directly but needs to transform into ammonium and nitrate first. transformation can done bacteria through enzymatic process. research aims obtain bacterial isolates fix nitrogen. Nitrogen-fixing were isolated using Nutrient Agar (NA) medium furthered nitrogenase activity detection test with semi-solid Free Bromothymol Blue (NFB). are characterized color changes medium. The results obtained 22 3 detected capable producing enzymes, namely TBP B3, TB1 B2, TMA2 B2.